Human monoclonal antibodies directed in opposition to the transmembrane glycoprotein (GP41) of human immunodeficiency virus-1 (HIV-1)
A way for neutralizing HIV-1 is disclosed. A most popular embodiment makes use of a novel human monoclonal antibody that binds to a conserved area of the gp41 transmembrane subunit of the virus.
The antibody is produced by steady cell traces developed utilizinghuman B lymphocyte cells collected from a affected person possessing excessive titers of anti-HIV antibodies.
The conserved area sure by the neutralizing antibody is a peptide of roughly 12 amino acids in size and ideally 8-10 amino acids. Related areas on HIV-2 and SIV are additionally disclosed.
The current invention is additional directed to a package and a way for detecting the presence and figuring out focus of an antibody that inhibits HIV-1 fusion-associated epitope, a peptide on gp41 with the amino acid sequence represented by GCSGKLIC.
kits-elisa
The detection and quantitation technique consists of an enzyme-linked immunosorbent assay (ELISA).
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
HIV-1 [HIV-1/Clade C (16055)] GP120 Protein, His Tag
Description: HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (GP5-V15224) is expressed from human 293 cells (HEK293). It contains AA Thr 34 - Arg 506 (Accession # ABL67444.1).
Description: HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag (GP4-V15223) is expressed from human 293 cells (HEK293). It contains AA Thr 36 - Lys 511 (Accession # Q4QX31).
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade B (89BZ_167)]
Description: Recombinant HIV glycoprotein 120 (amino acids 31-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade B (89BZ_167)]
Description: Recombinant HIV glycoprotein 120 (amino acids 31-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
HIV-1 [HIV-1/Clade B/C (CN54)] GP120 Protein, His Tag (MALS verified)
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
HIV-1 [HIV-1/Clade B/C (CN54)] GP120 Protein, His Tag (MALS verified)
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
The willpower of the focus of the antibody that inhibits HIV-1 fusion-associated epitope on gp41,current in physique fluids of a affected person seropositive for HIV-1 antibodies, supplies the doctor with an goal means to kind a prognosis for every particular person case and assists the clinician subsequently in figuring out the appropriateness to provoke medical intervention or change remedy.
Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed
Gastrin hormone immunoassays
The invention supplies assay strategies for the detection and quantitationof gastrin hormones, together with whole and free gastrin hormone in a pattern.
ELISA-type heterogeneous section assays appropriate to be used with organic fluidsamples comparable to blood, plasma or different bodily fluids of a mammal, notably a human topic are supplied.
The technique supplies a exact assayfor the quantities of free and whole G17 and G34 in organic fluid samples, in addition to the quantities of free and whole Gly-extended G17, and Gly-extended G34. Additionally supplied are strategies of figuring out appropriate therapy for affected person affected by a gastrin hormone-mediated illness or situation using gastrin hormone immunoassays.
Rabbit monoclonal antibodies in opposition to mouse/human Id3 proteins
The current invention pertains to a rabbit monoclonal antibody that binds to human Id3 protein and/or mouse Id3 protein with excessive specificity and excessive affinity.
The antibody has a binding fixed, measured with respect to human Id3 protein or mouse Id3, of better than 1.occasions.10.sup.8/molar. The antibody has no substantial cross-reactivity to different household Id proteins comparable to Id1, Id2, or Id4, or different endogenous proteins current within the cells that specific Id3 protein.
The specificityand excessive affinity of the rabbit monoclonal antibodies of the current invention permits delicate and particular detection and/or quantitation of human or mouse Id3 protein in organic samples.
The antibodies are helpful in immunochemical-based assays comparable to ELISA, western blot, and immunohistochemical staining.
Quantitative assay for human terminal complement cascade activation
The current invention discloses an enzyme-linked immunosorbent assay (ELISA) to quantitate fluid section terminal complement activation.
Upon activation to kind C5b-9, terminal complement parts specific neoantigens not current within the unassembled particular person parts.
Rabbit antiserum to polymerized C9 was rendered particular for C9 neoantigenic determinants by serial immunosorbtion with human serum, human C9, and different terminal complement parts sure to Sepharose.
Sum Formula: C117H211N41O31S; CAS# [198636-94-1] net
Utilizing the IgG from this antiserum, a sandwich ELISA was devised to bind SC5b-9 from resolution onto polystyrene plates.
The ELISA plates had been developed with using goat antiserum to native C9 epitopes adopted by a swine anti-goat IgG alkaline phosphatase conjugate.
Quantitation of SC5b-9 in resolution was carried out by evaluating pattern OD to a regular curve generated with human SC5b-9 that was purified from zymosan-activated serum.
The assay is delicate to as little as 100 ng of SC5b-9/ml and is helpful for screening plasma, serum, cerebrospinal fluid, or different organic fluids for the presence of terminal complement cascade activation.
Description: HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (GP5-V15224) is expressed from human 293 cells (HEK293). It contains AA Thr 34 - Arg 506 (Accession # ABL67444.1).
Description: HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag (GP4-V15223) is expressed from human 293 cells (HEK293). It contains AA Thr 36 - Lys 511 (Accession # Q4QX31).
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade A (KNH1144)]
Description: Recombinant HIV glycoprotein 120 (amino acids 36-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade A (KNH1144)]
Description: Recombinant HIV glycoprotein 120 (amino acids 36-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
gp120 (ADA) (HIV-1/Clade B) (aa 34-518), monomer only
Description: Recombinant HIV glycoprotein 120 (amino acids 36-506) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade C (16055)]
Description: Recombinant HIV glycoprotein 120 (amino acids 36-506) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
HIV-1 gp120 (6535) (Clade B) protein (amino acid 34-518)
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
HIV-1 [HIV-1/Clade B/C (CN54)] GP120 Protein, His Tag (MALS verified)
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
Description: Recombinant HIV glycoprotein 120 (amino acids 31-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade B (89BZ_167)]
Description: Recombinant HIV glycoprotein 120 (amino acids 31-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
HIV-1 gp120 (89.6P) (Clade B) protein (amino acid 34-518)
Description: Recombinant HIV glycoprotein 120 (amino acids 36-505) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade AE (CM240)]
Description: Recombinant HIV glycoprotein 120 (amino acids 36-505) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
HIV-1 gp120 (PVO) (Clade B) protein (amino acid 34-518)
