Human monoclonal antibodies directed in opposition to the transmembrane glycoprotein (GP41) of human immunodeficiency virus-1 (HIV-1)
A means for neutralizing HIV-1 is disclosed. A hottest embodiment makes use of a novel human monoclonal antibody that binds to a conserved space of the gp41 transmembrane subunit of the virus.
The antibody is produced by regular cell traces developed usinghuman B lymphocyte cells collected from a affected individual possessing extreme titers of anti-HIV antibodies.
The conserved space positive by the neutralizing antibody is a peptide of roughly 12 amino acids in dimension and ideally 8-10 amino acids. Associated areas on HIV-2 and SIV are moreover disclosed.
HIV (gp120) Antigenic Peptide | HIV-gp120-41-C
Sum Formula: C117H211N41O31S; CAS# [198636-94-1] net
The present invention is extra directed to a package deal and a means for detecting the presence and determining focus of an antibody that inhibits HIV-1 fusion-associated epitope, a peptide on gp41 with the amino acid sequence represented by GCSGKLIC.
kits-elisa
The detection and quantitation method consists of an enzyme-linked immunosorbent assay (ELISA).
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
HIV-1 [HIV-1/Clade C (16055)] GP120 Protein, His Tag
Description: HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (GP5-V15224) is expressed from human 293 cells (HEK293). It contains AA Thr 34 - Arg 506 (Accession # ABL67444.1).
Description: HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag (GP4-V15223) is expressed from human 293 cells (HEK293). It contains AA Thr 36 - Lys 511 (Accession # Q4QX31).
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade B (89BZ_167)]
Description: Recombinant HIV glycoprotein 120 (amino acids 31-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade B (89BZ_167)]
Description: Recombinant HIV glycoprotein 120 (amino acids 31-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
HIV-1 [HIV-1/Clade B/C (CN54)] GP120 Protein, His Tag (MALS verified)
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
HIV-1 [HIV-1/Clade B/C (CN54)] GP120 Protein, His Tag (MALS verified)
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
The willpower of the focus of the antibody that inhibits HIV-1 fusion-associated epitope on gp41,present in physique fluids of a affected individual seropositive for HIV-1 antibodies, provides the physician with an aim means to form a prognosis for each explicit individual case and assists the clinician subsequently in determining the appropriateness to impress medical intervention or change treatment.
Recombinant HIV-1 gp120 MN | HIV -gp120- Antigenic Peptide
Gastrin hormone immunoassays
The invention provides assay methods for the detection and quantitationof gastrin hormones, along with entire and free gastrin hormone in a sample.
ELISA-type heterogeneous part assays applicable for use with natural fluidsamples similar to blood, plasma or totally different bodily fluids of a mammal, notably a human subject are equipped.
The method provides a precise assayfor the portions of free and entire G17 and G34 in natural fluid samples, along with the portions of free and entire Gly-extended G17, and Gly-extended G34. Moreover equipped are methods of determining applicable remedy for affected individual affected by a gastrin hormone-mediated sickness or state of affairs utilizing gastrin hormone immunoassays.
Rabbit monoclonal antibodies in opposition to mouse/human Id3 proteins
The present invention pertains to a rabbit monoclonal antibody that binds to human Id3 protein and/or mouse Id3 protein with extreme specificity and extreme affinity.
The antibody has a binding mounted, measured with respect to human Id3 protein or mouse Id3, of higher than 1.events.10.sup.8/molar. The antibody has no substantial cross-reactivity to totally different family Id proteins similar to Id1, Id2, or Id4, or totally different endogenous proteins present throughout the cells that particular Id3 protein.
The specificityand extreme affinity of the rabbit monoclonal antibodies of the present invention permits delicate and explicit detection and/or quantitation of human or mouse Id3 protein in natural samples.
The antibodies are useful in immunochemical-based assays similar to ELISA, western blot, and immunohistochemical staining.
Quantitative assay for human terminal complement cascade activation
The present invention discloses an enzyme-linked immunosorbent assay (ELISA) to quantitate fluid part terminal complement activation.
Upon activation to form C5b-9, terminal complement elements particular neoantigens not present throughout the unassembled explicit individual elements.
Rabbit antiserum to polymerized C9 was rendered explicit for C9 neoantigenic determinants by serial immunosorbtion with human serum, human C9, and totally different terminal complement elements positive to Sepharose.
Recombinant HIV-1 TAT Clade-B | Recombinant HIV-1 nef , Clade B
Using the IgG from this antiserum, a sandwich ELISA was devised to bind SC5b-9 from decision onto polystyrene plates.
The ELISA plates had been developed with utilizing goat antiserum to native C9 epitopes adopted by a swine anti-goat IgG alkaline phosphatase conjugate.
Quantitation of SC5b-9 in decision was carried out by evaluating sample OD to a daily curve generated with human SC5b-9 that was purified from zymosan-activated serum.
The assay is delicate to as little as 100 ng of SC5b-9/ml and is useful for screening plasma, serum, cerebrospinal fluid, or totally different natural fluids for the presence of terminal complement cascade activation.
Description: HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (GP5-V15224) is expressed from human 293 cells (HEK293). It contains AA Thr 34 - Arg 506 (Accession # ABL67444.1).
Description: HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag (GP4-V15223) is expressed from human 293 cells (HEK293). It contains AA Thr 36 - Lys 511 (Accession # Q4QX31).
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade A (KNH1144)]
Description: Recombinant HIV glycoprotein 120 (amino acids 36-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade A (KNH1144)]
Description: Recombinant HIV glycoprotein 120 (amino acids 36-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
gp120 (ADA) (HIV-1/Clade B) (aa 34-518), monomer only
Description: Recombinant HIV glycoprotein 120 (amino acids 36-506) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade C (16055)]
Description: Recombinant HIV glycoprotein 120 (amino acids 36-506) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
HIV-1 gp120 (6535) (Clade B) protein (amino acid 34-518)
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
HIV-1 [HIV-1/Clade B/C (CN54)] GP120 Protein, His Tag (MALS verified)
Description: HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag derived from the env. gene of HIV-1 strain CN54 gp160 (Accession # G4XFJ5-1 (E46G, T396A, A497T), Thr 36 - Arg 507) and glycosylated with N-linked sugars and expressed in HEK293 cells at ACRObiosystems.
Description: Recombinant HIV glycoprotein 120 (amino acids 31-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade B (89BZ_167)]
Description: Recombinant HIV glycoprotein 120 (amino acids 31-511) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
HIV-1 gp120 (89.6P) (Clade B) protein (amino acid 34-518)
Description: Recombinant HIV glycoprotein 120 (amino acids 36-505) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
Human Immunodeficiency Virus GP120 Protein [HIV-1/Clade AE (CM240)]
Description: Recombinant HIV glycoprotein 120 (amino acids 36-505) produced in HEK293 cells. C-terminal transmembrane domain and the intravirion tail of the protein have been replaced by a 9 amino acid glycine-serine linker and a 6x histidine tag.
HIV-1 gp120 (PVO) (Clade B) protein (amino acid 34-518)
